The Rig-I/DDX58 Monoclonal Antibody (CAB23179) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets the RIG-I/DDX58 protein in human samples, making it a reliable tool for various research applications.RIG-I/DDX58 is a key sensor of viral RNA in the cytoplasm, triggering a cascade of signaling events that lead to the production of antiviral proteins and the induction of an immune response. By targeting RIG-I/DDX58 with this antibody, researchers can study the activation and regulation of this signaling pathway in different cell types, providing insights into the mechanisms of antiviral immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Rig-I/DDX58 Monoclonal Antibody
SKU:
CAB23179
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54108
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method
Affinity purification
Gene ID
23586
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from rat liver, using Rig-I/DDX58 Rabbit mAb (CAB23179) at 1:4000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from RAW 264.7 cells using Rig-I/DDX58 Rabbit mAb (CAB23179) at 1:4000 dilution incubated overnight at 4℃. RAW 264.7 cells were treated with LPS (1 μg/mL) at 37℃ overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
