The RNASE13 Antibody (CAB1073) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable nucleic acid binding activity. Predicted to be located in extracellular region.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RNASE13 Antibody
SKU:
CAB1073
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAL1, HEL-S-86p, RNASE13
Positive Sample:
Raji
Cellular Localization:
Secreted.
Calculated MW:
18kDa
Observed MW:
18kDa
Predicted to enable nucleic acid binding activity. Predicted to be located in extracellular region.
Purification Method
Affinity purification
Gene ID
440163
RRID
AB_2758182
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using RNASE13 Rabbit pAb (CAB1073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human prostate using RNASE13 Rabbit pAb (CAB1073) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using RNASE13 Rabbit pAb (CAB1073) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
