The RNF125 Antibody (CAB15165) is a high-quality antibody developed for reliable detection and analysis of target proteins. Produced in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the RNF125 protein, this antibody allows for the detection and analysis of RNF125 in various cell types, making it an essential reagent for studies in immunology and cancer research.RNF125, a ubiquitin ligase, plays a crucial role in the degradation of certain proteins involved in immune signaling pathways, making it a key player in the regulation of inflammatory responses and immune system function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RNF125 Antibody
SKU:
CAB15165
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TNORS, TRAC1, TRAC-1, RNF125
Positive Sample:
SK-MEL-28, U-937
Calculated MW:
26kDa
Observed MW:
26kDa
This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.
Purification Method
Affinity purification
Gene ID
54941
RRID
AB_2762054
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RNF125 Rabbit pAb (CAB15165) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
