The RNF126 Polyclonal Antibody (CAB24515) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the RNF126 protein, this antibody enables the detection and analysis of RNF126 in different cell types, making it an ideal choice for studies in cell biology and cancer research.RNF126, a member of the ring finger protein family, plays a crucial role in protein degradation pathways and the regulation of cellular homeostasis. Its involvement in protein quality control and turnover processes makes it a key player in maintaining cellular health and responding to cellular stress.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF126 Polyclonal Antibody
SKU:
CAB24515
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RNF126, ring finger protein 126
Positive Sample:
293T, Hep G2
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
33kDa/35kDa
Observed MW:
36kDa
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.
Purification Method
Affinity purification
Gene ID
55658
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RNF126 Rabbit pAb (CAB24515) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human liver using RNF126 Rabbit pAb (CAB24515) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
