The RNF185 Antibody (CAB16158) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blot applications.RNF185, also known as RING finger protein 185, is involved in the ubiquitin-proteasome pathway, where it plays a critical role in tagging proteins for degradation and maintaining cellular homeostasis. Dysregulation of RNF185 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular health and disease progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF185 Antibody
SKU:
CAB16158
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in positive regulation of ERAD pathway; protein autoubiquitination; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum.
Purification Method
Affinity purification
Gene ID
91445
RRID
AB_2763606
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse testis, using RNF185 Rabbit pAb (CAB16158) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
