The RING1B/RNF2 Antibody (CAB5563) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RNF2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and developmental biology.RNF2, also known as Ring1B, is a critical component of polycomb repressive complex 1 (PRC1) and plays a crucial role in epigenetic regulation and cell fate determination.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RING1B/RNF2 Antibody
SKU:
CAB5563
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
Purification Method
Affinity purification
Gene ID
6045
RRID
AB_2766343
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using RING1B/RNF2 Rabbit pAb (CAB5563) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using RING1B/RNF2 Rabbit pAb (CAB5563) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using RING1B/RNF2 Rabbit pAb (CAB5563) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg RING1B/RNF2 antibody (CAB5563). Western blot was performed from the immunoprecipitate using RING1B/RNF2 antibody (CAB5563) at a dilution of 1:1000.
