The RNF39 Polyclonal Antibody (PAC056964) is a valuable tool for researchers studying RNF39, a protein involved in the regulation of immune responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to RNF39, researchers can effectively detect and analyze this protein in various cell types, making it an ideal choice for studies in immunology and cancer research.RNF39, also known as a E3 ubiquitin-protein ligase, plays a crucial role in modulating immune responses by regulating the degradation of specific proteins involved in immune signaling pathways.
Understanding the function of RNF39 is essential for unraveling its role in diseases such as cancer, autoimmune disorders, and inflammatory conditions. By investigating the activity of RNF39, researchers can potentially uncover new therapeutic targets for manipulating immune responses in these disease contexts.
Immunofluorescence staining of HepG2 cells with PACO56964 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO56964 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May play a role in prolonged long term-potentiation (LTP) maintenance.
Synonyms:
RING finger protein 39 (Protein HZFw), RNF39, HZFW
UniProt Protein Function:
May play a role in prolonged long term-potentiation (LTP) maintenance.
UniProt Protein Details:
NCBI Summary:
This gene lies within the major histocompatibility complex class I region on chromosome 6. Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
