The RNF40 Antibody (CAB6443) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high specificity and sensitivity when used with human samples, and has been validated for use in Western blot applications. By binding to RNF40, the antibody enables researchers to detect and analyze this protein in various cell types, making it ideal for studies in molecular biology and cancer research.RNF40 is a crucial component of the ubiquitin-proteasome system, where it plays a role in targeting proteins for degradation and in regulating gene transcription.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RNF40 Antibody
SKU:
CAB6443
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRE1B, RBP95, STARING, RNF40
Positive Sample:
MCF7, HepG2, U-937
Cellular Localization:
Nucleus.
Calculated MW:
114kDa
Observed MW:
130kDa
The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9810
RRID
AB_2767045
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RNF40 Rabbit pAb (CAB6443) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
