The RNF8 Polyclonal Antibody (CAB7302) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RNF8 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for investigations in the fields of DNA repair, genomics, and cancer biology.RNF8 plays a crucial role in orchestrating the recruitment of DNA repair factors to sites of DNA damage, ultimately facilitating the repair of DNA lesions and maintenance of genomic integrity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF8 Polyclonal Antibody
SKU:
CAB7302
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
hRNF8, RNF8
Positive Sample:
HeLa, Rat brain
Cellular Localization:
Chromosome, Midbody, Nucleus, Telomere.
Calculated MW:
56kDa
Observed MW:
60kDa
The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9025
RRID
AB_2767843
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RNF8 Rabbit pAb (CAB7302) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
