The ROBO1 Antibody (CAB15313) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for human samples, making it an ideal choice for studies focusing on neural development and neural disorders.ROBO1 is a crucial player in guiding neuronal axons during development, ensuring precise connections between neurons in the brain and spinal cord. Dysregulation of ROBO1 has been implicated in various neurological disorders, making it a valuable target for research in neuroscience and neurodevelopmental disorders. With its high reactivity and specificity, the ROBO1 Polyclonal Antibody enables researchers to study ROBO1 expression and function in various cell types and tissues, providing valuable insights into neural development and potential therapeutic targets for neurological diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ROBO1 Antibody
SKU:
CAB15313
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NORS, NYS8, SAX3, CPHD8, DUTT1, ROBO1
Positive Sample:
293T, U-251MG, HeLa, 293T, U-251MG, HeLa, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Axon.
Calculated MW:
181kDa
Observed MW:
270kDa
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6091
RRID
AB_2762214
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ROBO1 Rabbit pAb (CAB15313) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates using ROBO1 Rabbit pAb (CAB15313) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
