The RPAP2 Polyclonal Antibody (CAB18500) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting RPAP2 in human samples, making it ideal for use in Western blot applications. By targeting the RPAP2 protein, researchers can gain insights into its function and involvement in various cellular processes, including gene expression and RNA metabolism.RPAP2 is a key player in the assembly of the RNA polymerase II complex, which is essential for transcribing genes and regulating gene expression. Dysregulation of RPAP2 has been linked to abnormal transcription activity and altered gene expression patterns, highlighting its importance in cellular function and disease development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RPAP2 Polyclonal Antibody
SKU:
CAB18500
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Rtr1, C1orf82, RPAP2
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Cytosol, Nucleolus, Nucleoplasm, Nucleus, Rna Polymerase Ii, Holoenzyme.
Calculated MW:
70kDa
Observed MW:
70kDa
Enables RNA polymerase II CTD heptapeptide repeat phosphatase activity. Involved in dephosphorylation of RNA polymerase II C-terminal domain and snRNA transcription. Located in cytosol and nucleolus. Part of RNA polymerase II, holoenzyme.
Purification Method
Affinity purification
Gene ID
79871
RRID
AB_2862264
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using RPAP2 Rabbit pAb (CAB18500) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
