The RPIA Antibody (CAB8867) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the RPIA protein, this antibody allows for the detection and analysis of RPIA levels in various cell types, making it ideal for research in areas such as metabolism, cancer, and cellular stress response.RPIA plays a crucial role in cellular energy metabolism by catalyzing the conversion of ribose-5-phosphate to ribulose-5-phosphate.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPIA Antibody
SKU:
CAB8867
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPI, RPIAD, RPIA
Positive Sample:
B cells, HL-60, Mouse thymus, Rat fat, Mouse spleen, Mouse skeletal muscle, Rat thymus
Cellular Localization:
Cytosol.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.
Purification Method
Affinity purification
Gene ID
22934
RRID
AB_2772070
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RPIA Rabbit pAb (CAB8867) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using RPIA Rabbit pAb (CAB8867) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
