The RPP40 Antibody (CAB17086) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Part of multimeric ribonuclease P complex.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPP40 Antibody
SKU:
CAB17086
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RNASEP1, bA428J1.3, RPP40
Positive Sample:
U-251MG, U-87MG, Mouse testis, Mouse kidney, Mouse liver, Rat lung, Rat thymus
Cellular Localization:
Nucleolar Ribonuclease P Complex, Nucleoplasm, Nucleus.
Calculated MW:
42kDa
Observed MW:
42kDa
Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Part of multimeric ribonuclease P complex.
Purification Method
Affinity purification
Gene ID
10799
RRID
AB_2772092
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPP40 Rabbit pAb (CAB17086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
