The S6 Ribosomal Protein (RPS6) Antibody (CAB6058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.RPS6 is a critical component of the ribosome and its phosphorylation status serves as a key regulator of translation initiation in response to various extracellular signals. The RPS6 Polyclonal Antibody binds specifically to RPS6, allowing for the detection and analysis of RPS6 in various cell types and tissues.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
S6 Ribosomal Protein (RPS6) Antibody
SKU:
CAB6058
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
S6, eS6, S6 Ribosomal Protein (RPS6)
Positive Sample:
SKOV3, Raji, HT-29, A-431, Mouse heart
Cellular Localization:
Cytoplasm, Cytoplasmic Ribonucleoprotein Granule, Cytosol, Cytosolic Ribosome, Endoplasmic Reticulum, Nucleolus, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm.
Calculated MW:
29kDa
Observed MW:
32kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method
Affinity purification
Gene ID
6194
RRID
AB_2766731
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using S6 Ribosomal Protein (RPS6) Rabbit pAb (CAB6058) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using S6 Ribosomal Protein (RPS6) Rabbit pAb (CAB6058) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
