The RRBP1 Antibody (CAB12239) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the RRBP1 protein, this antibody enables the detection and analysis of RRBP1 in various cell types, making it ideal for investigations in cell biology and molecular biology research.RRBP1, also known as ribosome binding protein 1, is essential for the proper functioning of cellular processes such as protein synthesis and intracellular trafficking.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RRBP1 Antibody
SKU:
CAB12239
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RRp, hES, p180, ES130, ES/130, RRBP1
Positive Sample:
U-87MG, HeLa, Mouse brain
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type Iii Membrane Protein.
Calculated MW:
152kDa
Observed MW:
120kDa
This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12.
Purification Method
Affinity purification
Gene ID
6238
RRID
AB_2759111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RRBP1 Rabbit pAb (CAB12239) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal immunofluorescence analysis of HeLa cells using RRBP1 Rabbit pAb (CAB12239) at dilution of 1:100. Blue: DAPI for nuclear staining.
