The MRP8 Monoclonal Antibody (CAB12018) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is produced in rabbits and has been shown to be highly specific and reactive in human samples, making it an excellent choice for studies in immunology and inflammation.S100A8 is a key player in the immune response, activating inflammatory pathways and promoting tissue damage in various diseases, including cancer and inflammatory disorders. By targeting and detecting S100A8 with this antibody, researchers can gain valuable insights into the role of this protein in disease progression and potentially identify new therapeutic targets.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRP8 Monoclonal Antibody
SKU:
CAB12018
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0478
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6279
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using S100A8 Rabbit mAb (CAB12018) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): C2C12. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using S100A8 Rabbit mAb (CAB12018) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
