The SAP130 Antibody (CAB13897) is a high-quality antibody developed for reliable detection and analysis of target proteins. SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]). RRID AB_2760751 Gene ID 79595 Swiss Prot Synonym SAP130
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SAP130 Antibody
SKU:
CAB13897
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SAP130
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
110kDa
Observed MW:
150kDa
SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]). RRID AB_2760751 Gene ID 79595 Swiss Prot Synonym SAP130
Purification Method:
Affinity purification
Gene ID:
79595
RRID:
AB_2760751
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using SAP130 Rabbit pAb (CAB13897) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
