The SARS-CoV-2 Spike RBD Antibody (CAB20135) is a high-quality antibody developed for reliable detection and analysis of target proteins. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. RRID AB_2862927 Gene ID 43740568 Swiss Prot Synonym spike glycoprotein; SARS-CoV-2 Spike RBD
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against SARS-CoV-2 samples.
Product Name:
SARS-CoV-2 Spike RBD Antibody
SKU:
CAB20135
Size:
100μL, 20μL
Reactivity:
SARS-CoV-2
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
1:50000-1:200000
Synonyms:
spike glycoprotein, SARS-CoV-2 Spike RBD
Positive Sample:
293T
Cellular Localization:
-
Calculated MW:
141kDa
Observed MW:
30kDa
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. RRID AB_2862927 Gene ID 43740568 Swiss Prot Synonym spike glycoprotein; SARS-CoV-2 Spike RBD
Purification Method:
Affinity purification
Gene ID:
43740568
RRID:
AB_2862927
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein,using SARS-CoV-2 Spike RBD Rabbit pAb (CAB20135) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of 293T-RBD and 293T cells using SARS-CoV-2 Spike RBD Rabbit pAb (CAB20135) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immobilized Recombinant SARS-COV-2 Spike RBD Protein (RP01278LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike RBD Rabbit pAb (CAB20135) with a linear range of 0.78-50ng/mL.
