The SCAP Polyclonal Antibody (PAC017050) is an essential tool for researchers studying SCAP, a key protein involved in cholesterol metabolism and regulation. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it a reliable choice for Western blot applications. By specifically binding to the SCAP protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for studies focused on lipid metabolism, cardiovascular health, and metabolic disorders.SCAP, or SREBP cleavage-activating protein, functions as a crucial mediator in the cellular response to cholesterol levels, playing a pivotal role in the regulation of lipid synthesis and homeostasis.
Understanding the molecular mechanisms of SCAP is fundamental for unraveling its contribution to metabolic diseases such as atherosclerosis, hypercholesterolemia, and non-alcoholic fatty liver disease. This antibody provides researchers with a valuable tool to explore the intricate pathways involving SCAP and its potential implications for therapeutic interventions targeting lipid-related disorders.
Antibody Name:
SCAP Antibody (PACO17050)
Antibody SKU:
PACO17050
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human SCAP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO17050(SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO17050(SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.
Synonyms:
SREBF chaperone
UniProt Protein Function:
SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 3p21.31Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membraneMolecular Function: protein binding; unfolded protein binding; cholesterol binding; protein complex bindingBiological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; response to hypoxia; positive regulation of low-density lipoprotein receptor biosynthetic process; SREBP-mediated signaling pathway; negative regulation of cholesterol biosynthetic process; response to insulin stimulus; aging
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq, Jul 2008]
sterol regulatory element-binding protein cleavage-activating protein
NCBI Synonym Full Names:
SREBF chaperone
NCBI Official Symbol:
SCAPÂ Â
NCBI Official Synonym Symbols:
NCBI Protein Information:
sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage-activating protein; sterol regulatory element binding protein cleavage-activating protein
UniProt Protein Name:
Sterol regulatory element-binding protein cleavage-activating protein
UniProt Synonym Protein Names:
Protein Family:
Sterol regulatory element-binding protein cleavage-activating protein
