The SCGB1A1 Antibody (CAB16997) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. RRID AB_2772153 Gene ID 7356 Swiss Prot Synonym UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP; SCGB1A1
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SCGB1A1 Antibody
SKU:
CAB16997
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UGB, UP1, CC10, CC16, CCSP, UP-1, CCPBP, SCGB1A1
Positive Sample:
Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
10kDa
Observed MW:
11kDa
This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. RRID AB_2772153 Gene ID 7356 Swiss Prot Synonym UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP; SCGB1A1
Purification Method:
Affinity purification
Gene ID:
7356
RRID:
AB_2772153
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse lung, using SCGB1A1 Rabbit pAb (CAB16997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded mouse lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.