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Flow cytometry stands as a pivotal technique in the realms of molecular biology and immunology, enabling the analysis of physical and chemical characteristics of cells or particles as they flow in

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Loading Control Antibodies | GAPDH, \u03b2-Actin & Tubulin | Assay Genie

Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Sweet Deception: How a New Antibody Therapy Unmasks Pancreatic Cancer Pancreatic canc

Antibody conjugation is a pivotal technique in biomedical research and diagnostic applications. This process involves the covalent attachment of a molecule, such as a drug, toxin, enzym

Techniques · Flow CytometryFlow Cytometry Gating: A Comprehensive GuideGating is how flow cytometry data is turned into meaningful results — a series of regions drawn on scatter and fluorescence plots

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In the evolving landscape of cellular biology, intracellular flow cytometry (ICFC) stands out as a powerful technique for analyzing the complexities within cells. This advanced method extends the

Immunophenotyping by Flow Cytometry Immunophenotyping is a technique used in the field of immunology and cell biology to identify and classify cells based on the specific proteins o

Lab Methods · Flow CytometryFlow Cytometry Protocol: Two Worked Protocols, Controls and 10 Practical TipsFlow cytometry measures individual cells in a fluid stream at hundreds per second, reporting si

Flow cytometry is a powerful analytical technology employed in cell biology, immunology, and oncology research. It enables the rapid measurement of physical and chemical characteristics of cells o

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Immunology · Antibody ProductionHybridoma Technology: How Monoclonal Antibodies Are MadeHybridoma technology, pioneered by Georges Köhler and César Milstein in 1975, is the method that made monoclonal

What does the COVID-19 Rapid POC CE-IVD Test detect? The test detects the presence of patient-generated antibodies against spike protein, the virus which causes the disease COVID-19. The tes

Antibodies · Reagent GuideMonoclonal vs Polyclonal Antibodies: Key DifferencesMonoclonal and polyclonal antibodies are the two workhorse reagent types in research, diagnostics and therapy. Monoclonals

Seroconversion Implications in SARS-CoV-2 Diagnostic Testing Key Points: COVID-19, the pandemic

Antibody Structure Antibody S

Quick Facts About FarletuzumabWhat is Farletuzumab?Farletuzumab is a humanized monoclonal antibody targeting folate receptor alpha (FRα), a protein overexpressed in ovarian and other epi

The selection of a secondary antibody is a critical step in the experimental design of various immunodetection methods, including Western Blot, ELISA, immunohistochemistry (IHC), and immunofluores

Immunodetection · ProtocolsSecondary Antibody Incubation: Times, Conditions and TroubleshootingThe secondary antibody step determines how much signal you get and how much background comes with it. Inc

In the realm of immunology, the terms "immunoglobulin" and "antibody" often find themselves used interchangeably, sparking confusion among those keen on understanding the immune system's fine deta

Antibodies, or immunoglobulins, stand as critical components of the immune system, orchestrating the identification and neutralization of pathogens like viruses and bacteria. This extensive artic

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Treponema pallidum, the causative agent of syphilis, triggers an immune response that produces antibodies detectable by laboratory tests. Diagnosing syphilis often involves detecting thes

Immunoglobulin antibodies stand at the forefront of the immune defense system, offering a sophisticated mechanism for recognizing and neutralizing a vast array of pathogens, including bacteria, v

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Immunology · Antibody BiologyAntibody Light Chains, Heavy Chains, Constant Regions & Tumour-Associated AntigensAn antibody’s specificity comes from its variable domains and its biological effect comes

In the evolving landscape of biochemical research and diagnostic assay development, the ability to label non-antibody proteins and small molecules with high precision has become indispensable. Thi

What You Need to Know About OzuriftamabWhat is Ozuriftamab?Ozuriftamab is an investigational monoclonal antibody targeting CD47, designed to enhance immune-mediated clearance of cancer c

1. What is a Biosimilar? A biosimilar is a biologic medical product that is highly similar to an already-approved reference biologic. While minor differences in clinically inactive compon

Quick Facts About LitifilimabWhat is Litifilimab?Litifilimab is a monoclonal antibody developed by Biogen targeting BDCA2, a receptor on plasmacytoid dendritic cells (pDCs), to regulate

CB6, also known as Etesevimab, is a monoclonal antibody that targets the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. It neutralizes the virus by preventing it from bind

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Flow Cytometry · Cell SortingGuide to Fluorescence-Activated Cell Sorting (FACS)Fluorescence-activated cell sorting (FACS) is a specialised form of flow cytometry that physically isolates individual c

T cell activation is a crucial process in the immune response, playing a pivotal role in how the body responds to pathogens and maintains immune homeostasis. This intricate process involves severa

Breakthrough Discovery: Epstein-Barr Virus Identified as Primary Trigger for Lupus A groundbreaking study published in November 2025 has provided the most definitive evidence to date that the common E

Immunophenotyping, a cornerstone of modern immunology, involves identifying and characterizing cell populations based on their surface markers. This technique provides insights into the heterog

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Immunology · Flow CytometryT Cell Immunophenotyping: Markers, Subsets and Panel DesignImmunophenotyping identifies cell populations by the proteins on their surface. For T cells that means resolving n

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Immunology Guide MHC Class I vs Class II: Key Differences & Functions [Guide] Major Histocompatibility Complex molecules present antigens to T

Immunology · ImmunophenotypingCD Markers on B Cells: Development, Subsets and ImmunophenotypingB cells change their surface marker profile at every stage from bone marrow progenitor to antibody-secret

Flow Cytometry · GatingExcluding Doublets in Flow Cytometry: Pulse Geometry and Gating StrategyTwo cells passing the laser together are recorded as one event, with roughly double the signal. In DNA co

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Immunology · Cell IsolationPeripheral Blood Mononuclear Cells (PBMCs): Composition, Isolation and UseMononuclear cells are the leukocytes with a single, unlobed nucleus — lymphocytes and monocytes. Is

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Cell Culture · ProtocolsCell Synchronisation Methods: Protocols for G1, S, G2 and M Phase ArrestAn asynchronous culture contains cells at every stage of the cycle at once, so any measurement is an ave

In the intricate landscape of the brain, cells constantly communicate, sending and receiving messages that govern our thoughts, memories, and movements. For decades, scientists believed this communica

What You Need to Know About MilatuzumabWhat is Milatuzumab?Milatuzumab is a humanized monoclonal antibody targeting CD74, a molecule involved in antigen presentation and cell signaling.W

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer The five main types of ELISA are sandwich, competitive, direct, indirect and multiplex. Sandwich ELISA is

Understanding PD-1 Blockade in B-Cell Lymphomas Understanding PD-1 Blockade in B-Cell Lymphomas Introduction The programmed cell death prote

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Immunology · Cell BiologyCells of the Innate Immune System: Types, Functions and MarkersThe innate immune system responds within minutes, without prior exposure, using cells that recognise conserved m

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What is Immunometabolism? Immunometabolism is an exponentially growing, multi-disciplinary field of research aiming at deciphering the dynamic cellular and mole

Immunology · Myeloid CellsGranulocytes: Neutrophils, Eosinophils and BasophilsGranulocytes are the leukocytes with cytoplasmic granules and lobed nuclei — neutrophils, eosinophils and basophils. They

Fibroblasts, essential components of connective tissues, play vital roles in maintaining tissue structure and function. Their remarkable ability to produce and organize the extracellular matrix

Immunology · HaematopoiesisMyeloid Lineage: Pioneers of Immune Cell DevelopmentThe myeloid lineage is one of the two great branches of haematopoiesis, generating the innate immune and blood cells — mo

Cell Signalling · CytokinesJAK-STAT Signalling: Mechanism, Regulation and InhibitorsJAK-STAT is the shortest route from a cytokine outside the cell to a change in gene transcription — receptor engagem

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Immunology · Antigen PresentationDendritic Cells: Markers, Activation and SubtypesDendritic cells are the professional antigen-presenting cells that decide whether an immune response happens at all. T

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~10 min read Quick Answer A sandwich ELISA uses two antibodies — a capture ant

Written by Seán Mac Fhearraigh, PhD  •  Updated: 19 May 2026  •  ~9 min read Quick Answer ELISA and Western Blot are both antibody-based protein detection methods. ELISA is faster, higher through

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Cell Signalling · MetabolismThe mTOR Signalling PathwaymTOR is a master regulator of cell growth, metabolism and survival, integrating nutrient, growth-factor and energy signals through two comp