Polyclonal Vs Monoclonal antibodies: Key features
In the field of immunology and biomedical research, antibodies play a crucial role in identifying and targeting foreign substances in the body. However, not all antibodies are the same. In this blog, we will explore the key differences between polyclonal and monoclonal antibodies, examining their production, characteristics, and implications in diagnostics, therapeutics, and research.
Key Takeaways
- Antibodies are essential in identifying and targeting foreign substances, with specificity governed by the lock-and-key mechanism.
- Monoclonal antibodies are highly specific, derived from a single cell clone, and utilized in targeted therapies and diagnostics.
- Polyclonal antibodies offer diverse binding specificities, enhancing sensitivity, and versatility in various research applications.
- Both types have advantages and are used in cancer therapy, research, and diagnostic tests, but may have different side effects.
What are Antigens and Antibodies?
Antigens are molecules that can stimulate an immune response, typically derived from pathogens such as bacteria, viruses, or foreign substances. On the other hand, antibodies, also known as immunoglobulins, are Y-shaped proteins produced by specialized white blood cells called B cells. Antibodies are designed to recognize and bind to specific antigens, marking them for destruction or neutralization
A schematic detailing the structure of an antibody
The interactions between antigens and antibodies are governed by a lock and key mechanism, where the unique structure of the antibody's binding site fits precisely with the complementary antigenic determinant, known as an epitope. This exquisite specificity allows antibodies to selectively recognize and bind to the corresponding antigens, akin to a lock and key fitting together perfectly. Upon binding, the antigen-antibody complex forms, triggering a cascade of events with profound implications for the immune response. Antigen-antibody interactions are not solely defined by the initial binding event. Factors such as affinity and avidity come into play, influencing the strength and stability of the complex formation. Moreover, these interactions are crucial for the development of immunological memory, allowing the immune system to mount a faster and more robust response upon subsequent encounters with the same antigen.
The Lock and Key Mechanism
The Art of Antibody Process Development
Selecting the Perfect Antigen
Antigens are molecules that trigger an immune response, leading to the generation of specific antibodies. Careful consideration is given to factors such as antigen purity, specificity, and immunogenicity. The choice of antigen is crucial, as it directly influences the success of subsequent steps in the production process.
Immunization
Immunization is a critical step in antibody production. It involves introducing the selected antigen into a host organism, such as a mouse or rabbit. This stimulates the immune system, leading to the production of antibodies against the target antigen. Adjuvants, substances that enhance the immune response, are often included to amplify the antibody generation process. The selection of an appropriate immunization protocol and monitoring of immune response kinetics are essential to obtain high-quality antibodies.
Harvesting the Antibody-Producing Cells
This step typically involves collecting blood or bone marrow samples from the immunized animal. The cells of interest, such as B lymphocytes, are isolated to obtain the specific antibodies generated against the antigen. Techniques like centrifugation and cell sorting aid in obtaining a pure population of antibody-producing cells.
In vitro Antibody Production
The isolated antibody-producing cells can be immortalized to create hybridoma cell lines or genetically engineered to express the desired antibody. These engineered cells can be grown in culture, allowing for the production of antibodies in a controlled environment. The culture medium, nutrient supply, and growth conditions are carefully optimized to achieve high antibody yields.
Antibody Purification and Quality Control
Purification is a critical step in antibody production, ensuring the isolation of high-quality antibodies from the complex mixture of culture supernatant or serum. Techniques like chromatography, filtration, and precipitation are employed to remove impurities and concentrate the antibodies. Quality control tests, such as SDS-PAGE, Western blotting, and ELISA, are performed to assess antibody purity, specificity, and functionality. Stringent quality control measures are implemented to ensure the final product meets the desired standards.
What is a recombinant protein?
A recombinant protein is a protein that has been created by combining two or more different proteins. The process of creating a recombinant protein is called recombination. Recombinant proteins are often used in research and medicine. For example, researchers might create a recombinant protein that contains the antibody portion of an antibody molecule and the receptor portion of a cell surface receptor molecule. This recombinant protein would then be able to attach to and neutralize an antigen.
Understanding Monoclonal Antibodies (mAbs): What Are They?
Immunoglobulins with a high degree of mono-specificity (for an antigen or epitope) are known as monoclonal antibodies. Monoclonal antibodies (mAbs) are laboratory-engineered proteins that mimic the immune system's natural ability to recognize and target specific substances, known as antigens. Unlike polyclonal antibodies derived from multiple sources, monoclonal antibodies are derived from a single clone of cells, making them highly precise in their binding capabilities.
Monoclonal antibodies consist of two fundamental components: a constant region (Fc) and a variable region (Fab). The Fab region exhibits exceptional specificity, recognizing and binding to specific target molecules, known as antigens, with remarkable precision. This antigen-binding capability forms the basis for targeted therapeutic interventions. Additionally, the Fc region governs the effector functions of monoclonal antibodies, such as recruitment of immune cells, complement activation, and antibody-dependent cellular cytotoxicity (ADCC).
Monoclonal antibodies exert their therapeutic effects through various mechanisms. One prominent mechanism involves neutralizing or blocking the target antigen, inhibiting its pathological effects. Another mechanism entails labeling the antigen for immune system recognition and subsequent destruction. Furthermore, monoclonal antibodies can interfere with cellular signaling pathways, modulate immune responses, or deliver payloads of drugs or radioactive substances specifically to diseased cells, minimizing damage to healthy tissues.
The production of monoclonal antibodies involves several key steps. It starts with the selection of an appropriate antigen, followed by immunization of laboratory animals, typically mice, with the chosen antigen. The animals' immune response leads to the production of antibodies. Next, scientists isolate antibody-producing cells from the animal and fuse them with immortalized cells, resulting in hybridoma cells that possess the ability to continuously produce identical monoclonal antibodies. These hybridoma cells are then cultured in the laboratory to generate a large quantity of monoclonal antibodies.
Steps involved in the production of mAbs
Understanding Polyclonal Antibodies (pAbs): What Are They?
Polyclonal antibodies (pAbs) are complex immune proteins derived from multiple B cell clones, each producing antibodies that recognize different epitopes of a specific antigen. Unlike monoclonal antibodies that originate from a single B cell clone, polyclonal antibodies offer a diverse repertoire of binding specificities. This characteristic makes them valuable for detecting various target molecules and enhances the sensitivity of immunodetection assays.
Structure of a Polyclonal Antibody (pAb)
Polyclonal antibodies are derived from a heterogeneous population of B cells, each producing a distinct antibody clone. This diversity arises from the body's natural immune response to antigens encountered during infections or immunizations. Unlike monoclonal antibodies that are produced by a single clone of cells, polyclonal antibodies comprise a mixture of different antibody classes (e.g., IgG, IgM, IgA) and subclasses, each with unique binding specificities. This broad repertoire allows polyclonal antibodies to recognize various epitopes on an antigen, increasing the likelihood of accurate and robust detection.
The mechanism of action of polyclonal antibodies involves the binding of multiple antibodies to different epitopes on an antigen. This multivalent binding enhances the sensitivity and avidity of antibody-antigen interactions, leading to more effective antigen recognition and neutralization. Polyclonal antibodies can engage in diverse immune functions, including opsonization, complement activation, antibody-dependent cellular cytotoxicity (ADCC), and antibody-dependent cell-mediated phagocytosis (ADCP). Such versatility makes polyclonal antibodies ideal for various research applications, including immunohistochemistry, flow cytometry, and Western blotting.
The production of polyclonal antibodies begins with the immunization of an animal, such as rabbits, goats, or mice, with the target antigen. This immunization stimulates the animal's immune system, triggering the production of a wide range of antibodies. Serum containing polyclonal antibodies is then collected from the immunized animal and undergoes purification to remove unwanted components. The resulting purified polyclonal antibodies can be further concentrated, characterized, and validated for their specificity and binding affinity.
What are the advantages of monoclonal vs polyclonal antibodies?
Monoclonal antibodies have several advantages over polyclonal antibodies. They are more specific and less likely to cause side effects. Additionally, monoclonal antibodies can be produced in large quantities. Further advantages of monoclonal antibodies include their high specificity, their long half-lives, and their low immunogenicity. However, monoclonal antibodies are more expensive to produce than polyclonal antibodies and they may not bind as well to an antigen. in addition monoclonal antibodies have a short shelf life, and must be injected.
Polyclonal antibodies have several advantages over monoclonal antibodies. They are more likely to bind to an antigen, they can bind to multiple epitopes on an antigen, and they can be produced more quickly. However, polyclonal antibodies are more likely to cause side effects, such as allergies, and they are less specific than monoclonal antibodies.
What are the uses of monoclonal and polyclonal antibodies?
The first human monoclonal antibody was produced in 1975 and since then monoclonal antibodies have been used in a wide range of applications including cancer therapy, autoimmune disease treatment, and diagnosis of infectious diseases. Polyclonal antibodies were first isolated in 1891 and they have a wide range of applications in research and medicine. Both monoclonal and polyclonal antibodies are often used to treat diseases, such as cancer, and they can be used in research. Additionally, monoclonal antibodies are sometimes used as drugs.
Monoclonal Antibodies
Product Name | Size | Reactivity |
- | Mouse | |
- | Mouse | |
120 µL- 200 µL | Human, Rat, Mouse |
Monoclonal antibodies as drugs
Monoclonal antibodies are sometimes used as drugs. For example, the monoclonal antibody rituximab is used to treat certain types of cancer. Omalizumab (brand name: Xolair) is a monoclonal antibody that targets the protein IgE, which is involved in allergies. Omalizumab is used to treat allergies. Rituximab (brand name: Rituxan) is a monoclonal antibody that targets the protein CD20, which is found on the surface of B cells. Rituximab is used to treat certain types of cancer, including leukemia, lymphoma, and solid tumors. Trastuzumab (Herceptin) is a monoclonal antibody that targets the protein HER2, which is found on the surface of some cancer cells. Trastuzumab is used to treat certain types of breast cancer and stomach cancer. Adalimumab (Humira) is a monoclonal antibody that targets the protein TNF, which is involved in inflammation. Adalimumab is used to treat a variety of inflammatory diseases, including Crohn's disease, psoriasis, and rheumatoid arthritis.
Cancer therapy
Monoclonal antibody therapy is a cancer treatment that uses monoclonal antibodies (mAbs) to target and destroy cancer cells. Monoclonal antibody therapy is used to treat a variety of cancers, including leukemia, lymphoma, and solid tumors. Monoclonal antibody therapy works by targeting specific proteins on the surface of cancer cells. These proteins are known as antigens. Monoclonal antibody therapy is used to treat cancer because it can specifically target and kill cancer cells without harming normal, healthy cells. Polyclonal antibody therapy is a treatment that uses polyclonal antibodies (pAbs) to target and destroy cancer cells. Polyclonal antibody therapy is used to treat a variety of cancers, including leukemia, lymphoma, and solid tumors. Polyclonal antibody therapy works by targeting specific proteins on the surface of cancer cells. These proteins are known as antigens. Polyclonal antibody therapy is used to treat cancer because it can specifically target and kill cancer cells without harming normal, healthy cells. The main difference between polyclonal antibody therapy and monoclonal antibody therapy is the type of antibody that is used. Monoclonal antibody therapy uses monoclonal antibodies, which are created by immune cells that have been exposed to only one antigen. This means that monoclonal antibody therapy is more specific than polyclonal antibody therapy. Additionally, monoclonal antibody therapy is more expensive than polyclonal antibody therapy. Polyclonal antibody therapy uses polyclonal antibodies, which are created by immune cells that have been exposed to multiple antigens. This means that polyclonal antibody therapy is less specific than monoclonal antibody therapy.
Immunofluorescence analysis of C6 cells using a-Tubulin Mouse mAb (CABC012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Research
Monoclonal and polyclonal antibodies are used in research. They can be used to study the structure and function of proteins, to purify proteins, and to detect proteins. Additionally, monoclonal and polyclonal antibodies can be used in diagnostic tests. For example, the PSA test, which is used to screen for prostate cancer, uses a monoclonal antibody that binds to the protein PSA.
Enzyme-linked Immunosorbent Assays (ELISA) use polyclonal or monoclonal antibodies to detect the presence of an antigen in a sample. ELISAs are used in a variety of settings, including medical diagnosis and research. ELISAs can be used to detect the presence of proteins, hormones, antibodies, and other molecules in a sample.
Immunohistochemistry (IHC) is a technique that uses antibodies to detect the presence of antigens in cells. IHC is used in medical diagnosis and research. IHC can be used to detect the presence of proteins, hormones, antibodies, and other molecules in cells.
Western blotting is a technique that uses antibodies to detect the presence of proteins in a sample. Western blotting is used in research and medical diagnosis. Western blotting can be used to detect the presence of proteins, hormones, antibodies, and other molecules in a sample.
Immunoprecipitation (IP) is a technique that uses antibodies to purify proteins from a sample. IP is used in research and medical diagnosis. IP can be used to purify proteins, hormones, antibodies, and other molecules from a sample.
What are the side effects of monoclonal and polyclonal antibody therapy?
The side effects of monoclonal antibody therapy can include fever, chills, nausea, vomiting, diarrhea, headache, muscle pain, and fatigue. The side effects of polyclonal antibody therapy can include fever, chills, nausea, vomiting, diarrhea, headache, and fatigue. In rare cases, both monoclonal and polyclonal antibody therapies can cause anaphylaxis. Anaphylaxis is a severe allergic reaction that can be life-threatening.
Polyclonal Antibodies
Product Name | Size | Reactivity |
120 µL | Human, Mouse, Rat | |
- | Human, Mouse, Rat | |
120 µL- 200 µL | Human, Rat, Mouse |
Key Differences : pAbs vs mAbs
Comparison Factors | pAbs | mAbs |
Origin | Derived from a diverse population of B cells | Derived from a single clone of B cells |
Speciicity | Recognize multiple epitopes on the antigen | Recognize a single epitope on the antigen |
Heterogenicity | Heterogeneous mixture of antibody clones | Homogeneous population of identical antibodies |
Binding Affinity | Varying binding affinities | Consistent and high binding affinity |
Production Process | Produced by immunzing animals and collecting serum | Produced through hybridoma technology or recombinant DNA technology |
Cross-Reactivity | Potential for crossreactivity with similar antigens | Minimal or no cross-reactivity with other antigens |
Quantity | Higher antibody yield | Limited antibody yield |
Cost | Geneally more cost-effective | Can be more expensive |
Applications | Broad range of applications, including diagnostics, research, and therapeutics | Well-suited for targeted therapies, diagnostics, and research requiring high specificity |
Advantages | Wide range of epitope recognition, robust detection and versatility | High specificty, reproducibilty and homogeneous population |
Disadvantages | Potential for non-specific binding and batch-to-batch variability | Limited epitope coverage, lower diversity, and potential immunogenecity |
Written by Pragna Krishnapur
Pragna Krishnapur completed her bachelor degree in Biotechnology Engineering in Visvesvaraya Technological University before completing her masters in Biotechnology at University College Dublin.
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