The SCG2 Antibody (CAB6425) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for Secretogranin-2 in human samples and has been validated for use in Western blot applications. By binding to the Secretogranin-2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in endocrinology, neurobiology, and cancer research.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
SCG2 Antibody
SKU:
CAB6425
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SN, CHGC, EM66, SgII, SCG2
Positive Sample:
SW620, Jurkat
Cellular Localization:
Secreted.
Calculated MW:
71kDa
Observed MW:
65kDa
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown.
Purification Method
Affinity purification
Gene ID
7857
RRID
AB_2767027
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SCG2 Rabbit pAb (CAB6425) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded human pancreas using SCG2 Rabbit pAb (CAB6425) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
