The SEMA6A Polyclonal Antibody (PAC020422) is a valuable tool for researchers studying SEMA6A, a member of the semaphorin family of proteins known to play a role in axon guidance and cell migration. This antibody, produced in-house, has been validated for use in various applications, including Western blot and immunohistochemistry, and shows high specificity in detecting SEMA6A in human samples.SEMA6A is involved in various biological processes, including neuronal development and immune response modulation.
Dysregulation of SEMA6A has been linked to neurological disorders and cancer progression, making it an important target for further investigation. The SEMA6A Polyclonal Antibody allows for precise detection and analysis of SEMA6A expression in different cell types, providing valuable insights for research in neuroscience and oncology.
Antibody Name:
SEMA6A Antibody (PACO20422)
Antibody SKU:
PACO20422
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human SEMA6A
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE,Lysate: 40 µg,Lane 1-3: 293T cells, Lovo cells, HepG2 cells,Primary antibody: PACO20422(SEMA6A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes.
Background:
The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system.
Synonyms:
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
UniProt Protein Function:
SEMA6A: a single-pass type I membrane protein of the semaphorin family that can act as repulsive axon guidance cues. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation. Active as a homodimer or oligomer. Interacts with EVL. Two isoforms of the human protein are produced by alternative splicing.Protein type: Cell adhesion; Membrane protein, integralChromosomal Location of Human Ortholog: 5q23.1Cellular Component: integral to plasma membraneMolecular Function: chemorepellent activity; protein binding; semaphorin receptor bindingBiological Process: negative regulation of axon extension involved in axon guidance; neural crest cell migration
UniProt Protein Details:
NCBI Summary:
The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]
