The Septin 8 Monoclonal Antibody (CAB21148) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 23176 Swiss Prot Synonym SEP2; SEPT8; Septin 8
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Septin 8 Monoclonal Antibody
SKU:
CAB21148
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2988
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SEP2, SEPT8, Septin 8
Positive Sample:
HeLa, K-562, Mouse liver, Mouse uterus, Rat heart
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
56kDa
Observed MW:
56kDa
This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 23176 Swiss Prot Synonym SEP2; SEPT8; Septin 8
Purification Method
Affinity purification
Gene ID
23176
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Septin 8 Rabbit mAb (CAB21148) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
