The PEDF/SERPINF1 Antibody (CAB1673) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. RRID AB_2763728 Gene ID 5176 Swiss Prot Synonym OI6; OI12; PEDF; EPC-1; PIG35; PEDF/SERPINF1
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PEDF/SERPINF1 Antibody
SKU:
CAB1673
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OI6, OI12, PEDF, EPC-1, PIG35, PEDF/SERPINF1
Positive Sample:
HepG2, Mouse liver, Human serum
Cellular Localization:
Melanosome, Secreted.
Calculated MW:
46kDa
Observed MW:
46kDa
This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. RRID AB_2763728 Gene ID 5176 Swiss Prot Synonym OI6; OI12; PEDF; EPC-1; PIG35; PEDF/SERPINF1
Purification Method:
Affinity purification
Gene ID:
5176
RRID:
AB_2763728
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PEDF/PEDF/SERPINF1 Rabbit pAb (CAB1673) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of various lysates using PEDF/PEDF/SERPINF1 Rabbit pAb (CAB1673) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
