The SGK3 Antibody (CAB7586) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity for human SGK3 and has been validated for use in Western blot applications.SGK3 is known to play a role in various cellular processes, including cell survival, proliferation, and metabolism. It is also involved in regulating ion channels and transporters, making it a key player in cellular signaling pathways. The SGK3 Polyclonal Antibody allows for the detection and analysis of SGK3 expression in different cell types, providing valuable insights into its function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SGK3 Antibody
SKU:
CAB7586
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CISK, SGK2, SGKL, SGK3
Positive Sample:
HL-60, THP-1, Mouse kidney
Cellular Localization:
Cytoplasmic Vesicle, Early Endosome, Recycling Endosome.
Calculated MW:
57kDa
Observed MW:
57kDa
This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
23678
RRID
AB_2768107
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SGK3 Rabbit pAb (CAB7586) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
