Description
SIRT3 Monoclonal Antibody (CAB22409)
The SIRT3 Monoclonal Antibody (CAB22409) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to target SIRT3 in human samples, allowing for precise detection and analysis in Western blot applications.SIRT3 is a member of the sirtuin family of proteins, known for their role in regulating energy metabolism and stress responses. As a mitochondrial deacetylase, SIRT3 plays a crucial role in maintaining mitochondrial function and protecting cells from oxidative damage. Dysregulation of SIRT3 has been linked to various age-related diseases, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | SIRT3 Monoclonal Antibody |
| SKU: | CAB22409 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC51535 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | QRFL LHVV DFPM ADLL LILG TSLE VEPF ASLT EAVR SSVP RLLI NRDL VGPL AWHP RSRD VAQL GDVV HGVE SLVE LLGW TEEM RDLV QRET GKLD GPDK | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | SIR2L3, T3 |
| Positive Sample: | 293T, Hep G2 |
| Cellular Localization: | Mitochondrion Matrix. |
| Calculated MW: | 44kDa |
| Observed MW: | 28kDa |
SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
| Purification Method | Affinity purification |
| Gene ID | 23410 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from Hep G2 cells using [KO Validated] SIRT3 Rabbit mAb (CAB22409) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and SIRT3 knockout (KO) 293T cells, using [KO Validated] SIRT3 Rabbit mAb (CAB22409) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s. |
