The SKIV2L2 Antibody (CAB5576) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples and has been validated for use in Western blot applications. By binding to the SKIV2L2 protein, this antibody allows for the detection and analysis of SKIV2L2 expression in various cell types, making it an essential tool for investigations in molecular biology and RNA biology.SKIV2L2 is a crucial component of the RNA exosome complex, which plays a vital role in degrading and processing RNA molecules within the cell.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SKIV2L2 Antibody
SKU:
CAB5576
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables ATP binding activity and RNA helicase activity. Involved in RNA catabolic process; cellular response to DNA damage stimulus; and maturation of 5.8S rRNA. Located in nucleoplasm. Part of TRAMP complex and catalytic step 2 spliceosome. Colocalizes with nuclear exosome (RNase complex). Biomarker of amyotrophic lateral sclerosis.
Purification Method
Affinity purification
Gene ID
23517
RRID
AB_2766353
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SKIV2L2 Rabbit pAb (CAB5576) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SKIV2L2 Rabbit pAb (CAB5576) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis using SKIV2L2 Rabbit pAb (CAB5576) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SKIV2L2 antibody (CAB5576). Western blot was performed from the immunoprecipitate using SKIV2L2 antibody (CAB5576) at a dilution of 1:500.
