The Smad5 Monoclonal Antibody (CAB22749) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, exhibits high specificity and sensitivity for detecting SMAD5 in human samples, making it ideal for use in Western blot and immunohistochemistry applications.SMAD5 is a transcription factor that plays a crucial role in cell growth, differentiation, and development. Dysregulation of the TGF-β signaling pathway, in which SMAD5 is involved, has been linked to various diseases, including cancer, fibrosis, and developmental disorders. By targeting SMAD5 with this monoclonal antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Smad5 Monoclonal Antibody
SKU:
CAB22749
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55344
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DWFC, JV5-1, MADH5, d5
Positive Sample:
293T, HeLa, C2C12, PC-12
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
52kDa
Observed MW:
60kDa
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4090
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T cells using [KO Validated] Smad5 Rabbit mAb (CAB22749) at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of various lysates using [KO Validated] Smad5 Rabbit mAb (CAB22749) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
