The [KO Validated] SMARCB1/SNF5 Antibody (CAB5767) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to SMARCB1, this antibody allows for the detection and analysis of this important protein in a variety of cell types, making it ideal for studies in epigenetics, cancer research, and developmental biology.SMARCB1, also known as INI1, is a key player in the SWI/SNF chromatin remodeling complex, which regulates gene expression by altering the structure of chromatin.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SMARCB1/SNF5 Antibody
SKU:
CAB5767
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
293T, NIH/3T3, Jurakt, K-562, Neuro-2a, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
44kDa
Observed MW:
47kDa
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
6598
RRID
AB_2766521
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SMARCB1/SNF5 Rabbit pAb (CAB5767) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human epithelioid sarcoma (ini-1 deletion) using SMARCB1/SNF5 Rabbit pAb (CAB5767) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg SMARCB1/SNF5 antibody (CAB5767). Western blot was performed from the immunoprecipitate using SMARCB1/SNF5 antibody (CAB5767) at a dilution of 1:1000.
