The SMG5 Antibody (CAB18123) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody specifically targets SMG5 in human samples and is recommended for use in Western blot applications. By binding to SMG5, researchers can study its expression levels and functions in various cell types, offering insights into RNA quality control mechanisms and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SMG5 Antibody
SKU:
CAB18123
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EST1B, SMG-5, LPTSRP1, LPTS-RP1, SMG5
Positive Sample:
Mouse spleen, Mouse brain
Cellular Localization:
Cytoplasm, Cytosol, Nucleus.
Calculated MW:
114kDa
Observed MW:
114kDa
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
Purification Method
Affinity purification
Gene ID
23381
RRID
AB_2861914
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMG5 Rabbit pAb (CAB18123) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
