The SMPD1/ASM Antibody (CAB6743) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMPD1/ASM Antibody
SKU:
CAB6743
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF/ICC
1:10 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASM, NPD, ASMASE, SMPD1 / ASM
Positive Sample:
HepG2, MCF7, Hep G2, U-251 MG, MCF7, Mouse liver, Rat brain
Cellular Localization:
Lysosome.
Calculated MW:
70kDa
Observed MW:
55kDa/70kDa/60kDa,70-75kDa
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
6609
RRID
AB_2767327
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMPD1 / ASM Rabbit pAb (CAB6743) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using SMPD1 / ASM Rabbit pAb (CAB6743). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
