The SMYD2 Antibody (CAB6474) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of SMYD2 expression in various cell types.SMYD2 is a lysine methyltransferase that plays a critical role in modulating gene expression and cell signaling pathways, making it a promising target for cancer therapy.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SMYD2 Antibody
SKU:
CAB6474
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KMT3C, HSKM-B, ZMYND14, SMYD2
Positive Sample:
Hep G2
Cellular Localization:
Cytoplasm, Nucleus, Cytosol.
Calculated MW:
50kDa
Observed MW:
50kDa
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).
Purification Method
Affinity purification
Gene ID
56950
RRID
AB_2767075
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells, using SMYD2 Rabbit pAb (CAB6474) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunofluorescence analysis of U2OS cells using SMYD2 Rabbit pAb (CAB6474). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
