The SMYD3 Antibody (CAB14516) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2761391 Gene ID 64754 Swiss Prot Synonym KMT3E; ZMYND1; ZNFN3A1; bA74P14.1; SMYD3
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMYD3 Antibody
SKU:
CAB14516
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KMT3E, ZMYND1, ZNFN3A1, bA74P14.1, SMYD3
Positive Sample:
22Rv1, HT-29, HepG2, MCF7, HeLa, Mouse testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2761391 Gene ID 64754 Swiss Prot Synonym KMT3E; ZMYND1; ZNFN3A1; bA74P14.1; SMYD3
Purification Method
Affinity purification
Gene ID
64754
RRID
AB_2761391
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMYD3 Rabbit pAb (CAB14516) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using SMYD3 Rabbit pAb (CAB14516) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using SMYD3 Rabbit pAb (CAB14516) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using SMYD3 Rabbit pAb (CAB14516) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
