The SNAP25 Monoclonal Antibody (CAB2234) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is particularly well-suited for use in immunohistochemistry and immunofluorescence applications. It binds specifically to the SNAP25 protein, allowing for precise visualization and analysis in various tissue samples and cell types.SNAP25, or synaptosome-associated protein 25, is essential for synaptic vesicle fusion and neurotransmitter release in neuronal cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SNAP25 Monoclonal Antibody
SKU:
CAB2234
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0736
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
6616
RRID
AB_2862979
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SNAP25 Rabbit mAb (CAB2234) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
