The SOCS2 Antibody (CAB17035) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is suitable for use in Western blot applications.SOCS2 is known for its ability to regulate immune responses by modulating the expression of cytokines and their downstream signaling molecules. Its involvement in various cellular processes, including growth, differentiation, and inflammation, makes it a promising target for studies in immunology and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SOCS2 Antibody
SKU:
CAB17035
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CIS2, SSI2, Cish2, SSI-2, SOCS-2, STATI2, SOCS2
Positive Sample:
A-549, 293T
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
22kDa
Observed MW:
22kDa
This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has pseudogenes on chromosomes 20 and 22. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8835
RRID
AB_2772341
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SOCS2 Rabbit pAb (CAB17035) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using SOCS2 Rabbit pAb (CAB17035) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
