The [KO Validated] SOD2 Antibody (CAB1340) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human, mouse, and rat samples, making it suitable for various experimental applications, including Western blot analysis.SOD2 is a critical enzyme that plays a key role in the regulation of reactive oxygen species (ROS) levels within cells, protecting them from oxidative damage and maintaining cellular homeostasis. Dysregulation of SOD2 has been implicated in a variety of diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SOD2 Antibody
SKU:
CAB1340
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
293T, HepG2, A-549, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain, Rat heart
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
25kDa
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Purification Method
Affinity purification
Gene ID
6648
RRID
AB_2760260
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using [KO Validated] SOD2 Rabbit pAb (CAB1340) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and SOD2 knockout (KO) 293T cells, using SOD2 Rabbit pAb (CAB1340) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using SOD2 Rabbit pAb (CAB1340) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SOD2 Rabbit pAb(CAB1340) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
