Description
SPAG1 Antibody (PACO18425)
The SPAG1 Polyclonal Antibody (PAC018425) is a valuable tool for researchers studying SPAG1, a protein involved in multiple cellular processes, including ciliary function and sperm motility. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the SPAG1 protein, this antibody enables accurate detection and quantification in a variety of cell types, making it suitable for investigations in developmental biology and reproductive health.
SPAG1 is a key player in flagellar assembly and function, making it essential for processes like ciliary beating and sperm motility. Research on SPAG1 is crucial for understanding the molecular mechanisms underlying diseases related to ciliary dysfunction, such as primary ciliary dyskinesia and male infertility. By elucidating the role of SPAG1 in these conditions, researchers can potentially identify novel therapeutic targets for intervention and treatment.
| Antibody Name: | SPAG1 Antibody (PACO18425) |
| Antibody SKU: | PACO18425 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide of human SPAG1 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO18425(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
 | Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human colon tissue, Primary antibody: PACO18425(SPAG1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18425(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200). |
| Background: | The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. |
| Synonyms: | Sperm associated antigen 1 |
| UniProt Protein Function: | SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA); Microtubule-binding Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm Molecular Function:GTP binding; hydrolase activity Biological Process: metabolic process; single fertilization Disease: Ciliary Dyskinesia, Primary, 28 |
| UniProt Code: | Q07617 |
| NCBI GenInfo Identifier: | 223634718 |
| NCBI Gene ID: | |
| NCBI Accession: | Q07617.3 |
| Molecular Weight: | 104kDa |
| NCBI Full Name: | Sperm-associated antigen 1 |
| UniProt Protein Name: | Sperm-associated antigen 1 |
| UniProt Synonym Protein Names: | HSD-3.8; Infertility-related sperm protein Spag-1 |
| Protein Family: | Sperm-associated antigen |
| UniProt Gene Name: | SPAG1Â Â |
| UniProt Entry Name: | SPAG1_HUMAN |
| ELISA Kits |
| Human SPAG1 / Sperm-associated antigen 1 ELISA Kit |
| Human Sperm-associated antigen 1 (SPAG1) ELISA Kit |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
