The SPATA18 Polyclonal Antibody (PAC020591) is a valuable tool for researchers studying SPATA18, a protein involved in spermatogenesis and male fertility. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the SPATA18 protein, this antibody enables detection and analysis in various cell types, making it ideal for studies in reproductive biology and male infertility research.SPATA18, also known as spermatogenesis-associated protein 18, is essential for normal sperm development and function. Understanding the role of SPATA18 is crucial for investigating male reproductive health and fertility issues.
Research on SPATA18 has potential implications for improving diagnosis and treatment of male infertility, as well as developing new therapies to overcome reproductive challenges.Overall, the SPATA18 Polyclonal Antibody is a reliable and sensitive tool for researchers interested in exploring the functions and mechanisms of SPATA18 in spermatogenesis and male fertility. Its specificity and versatility make it a valuable asset in advancing our understanding of reproductive biology and guiding the development of targeted therapies for male reproductive disorders.
Antibody Name:
SPATA18 Antibody (PACO20591)
Antibody SKU:
PACO20591
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human SPATA18
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20591(SPATA18 Antibody) at dilution 1/250 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes.
Background:
SPATA18 (spermatogenesis associated 18), also known as Mieap or SPETEX1, is a 538 amino acid, protein that is thought to play a role in cell differentiation during spermatogenesis, particularly during development from late elongate spematids to mature spermatozoa. Localizing to cytoplasm, SPATA18 is encoded by a gene that maps to human chromosome 4q12.
Synonyms:
spermatogenesis associated 18
UniProt Protein Function:
SPATA18: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Belongs to the MIEAP family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: MitochondrialChromosomal Location of Human Ortholog: 4q12Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; cytoplasmMolecular Function: protein bindingBiological Process: response to DNA damage stimulus
