The SPINT1 Antibody (CAB6411) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples, making it an ideal choice for Western blot applications.SPINT1, also known as HAI-1, is a potent inhibitor of certain proteases involved in cell growth and differentiation. Dysregulation of SPINT1 has been linked to various diseases, including cancer and inflammatory conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPINT1 Antibody
SKU:
CAB6411
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HAI, HAI1, MANSC2, SPINT1
Positive Sample:
Mouse liver, Rat brain
Cellular Localization:
Secreted.
Calculated MW:
58kDa
Observed MW:
70kDa
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
6692
RRID
AB_2767013
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPINT1 Rabbit pAb (CAB6411) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using SPINT1 Rabbit pAb (CAB6411). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
