The SPP1 Polyclonal Antibody (CAB1499) is a valuable tool for researchers studying SPP1, a protein involved in inflammation, immune response, and tissue repair. This antibody, generated in rabbits, has high specificity and sensitivity for detecting SPP1 in human samples, making it suitable for use in Western blot and immunohistochemistry applications.SPP1, also known as osteopontin, is a multifunctional protein that plays a role in various biological processes such as cell adhesion, migration, and survival.
Its expression is upregulated in cancer, inflammatory diseases, and tissue injury, making it a potential biomarker and therapeutic target for these conditions. The SPP1 Polyclonal Antibody enables researchers to study the expression and function of SPP1 in different cell types and tissues, providing valuable insights into its role in disease pathogenesis and potential therapeutic interventions.
Product Name:
Osteopontin Rabbit Polyclonal Antibody
SKU:
CAB1499
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 106-205 of human Osteopontin (NP_001035147.1).
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat kidney, using Osteopontin Rabbit pAb (CAB1499) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
