The SPTLC3 Polyclonal Antibody (PAC040658) is a valuable tool for researchers studying SPTLC3, a key enzyme involved in sphingolipid metabolism. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot applications. By binding to the SPTLC3 protein, this antibody enables accurate detection and analysis in various cell types, facilitating research in lipid metabolism and related diseases.SPTLC3 is a crucial enzyme in the biosynthesis of sphingolipids, which play essential roles in cell signaling, membrane structure, and lipid metabolism.
Dysregulation of sphingolipid metabolism has been linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer. Research on SPTLC3 is therefore important for understanding the molecular mechanisms underlying these conditions and developing targeted therapeutics. In conclusion, the SPTLC3 Polyclonal Antibody is a powerful tool for investigators in the field of lipid metabolism and related diseases. Its high specificity and reliability make it a valuable asset for studies aiming to unravel the complex roles of sphingolipids in health and disease.
Western blot. All lanes: SPTLC3 antibody at 0.2µg/ml. Lane 1: Mouse kidney tissue. Lane 2: U87 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63, 20 kDa. Observed band size: 63 kDa.
Immunohistochemistry of paraffin-embedded human colon cancer using PACO40658 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO40658 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.
Synonyms:
Serine palmitoyltransferase 3 (EC 2.3.1.50) (Long chain base biosynthesis protein 2b) (LCB2b) (Long chain base biosynthesis protein 3) (LCB 3) (Serine-palmitoyl-CoA transferase 3) (SPT 3), SPTLC3, C20orf38 SPTLC2L
UniProt Protein Function:
SPTLC3: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 2.3.1.50; Endoplasmic reticulum; Transferase; Membrane protein, integralChromosomal Location of Human Ortholog: 20p12.1Cellular Component: endoplasmic reticulum membraneMolecular Function: serine C-palmitoyltransferase activityBiological Process: sphingoid biosynthetic process; sphingolipid biosynthetic process
UniProt Protein Details:
NCBI Summary:
The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50), which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al., 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3), depending on the tissue in which biosynthesis occurs (Hornemann et al., 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit, SSSPTA (MIM 613540) and SSSPTB (MIM 610412), that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al., 2009 [PubMed 19416851]).[supplied by OMIM, Nov 2010]
