Description
Anti-ST14 Antibody (CAB13549)
The ST14 Polyclonal Antibody (CAB13549) is a valuable tool for researchers studying the ST14 protein, also known as matriptase or epithin. ST14 is a membrane-anchored serine protease implicated in various biological processes, including cell adhesion, migration, and proliferation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.The ST14 Polyclonal Antibody binds to the ST14 protein, enabling accurate detection and analysis in a variety of cell types.
Its high reactivity and specificity make it an ideal choice for immunology and cancer research studies focused on understanding the role of ST14 in tumorigenesis, metastasis, and other pathological processes. By targeting ST14, researchers can uncover valuable insights into potential therapeutic strategies for cancer and other diseases influenced by ST14 activity.
| Antibody Name: | Anti-ST14 Antibody |
| Antibody SKU: | CAB13549 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 566-855 of human ST14 (NP_068813.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 566-855 of human ST14 (NP_068813.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TCTK HTYR CLNG LCLS KGNP ECDG KEDC SDGS DEKD CDCG LRSF TRQA RVVG GTDA DEGE WPWQ VSLH ALGQ GHIC GASL ISPN WLVS AAHC YIDD RGFR YSDP TQWT AFLG LHDQ SQRS APGV QERR LKRI ISHP FFND FTFD YDIA LLEL EKPA EYSS MVRP ICLP DASH VFPA GKAI WVTG WGHT QYGG TGAL ILQK GEIR VINQ TTCE NLLP QQIT PRMM CVGF LSGG VDSC QGDS GGPL SSVE ADGR IFQA GVVS WGDG CAQR NKPG VYTR LPLF RDWI KENT GV |
| Gene ID: | 6768 |
| Uniprot: | Q9Y5Y6 |
| Cellular Location: | Membrane, Single-pass type II membrane protein |
| Calculated MW: | 94kDa |
| Observed MW: | 100kDa |
| Synonyms: | ST14, ARCI11, HAI, MT-SP1, MTSP1, PRSS14, SNC19, TADG15, TMPRSS14 |
| Background: | The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. |
| UniProt Protein Function: | ST14: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH). ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:EC 3.4.21.109; Membrane protein, integral; Protease Chromosomal Location of Human Ortholog: 11q24-q25 Cellular Component: extrinsic to plasma membrane; extracellular space; integral to plasma membrane; basolateral plasma membrane; plasma membrane Molecular Function:serine-type peptidase activity; serine-type endopeptidase activity Biological Process: keratinocyte differentiation; proteolysis Disease: Ichthyosis, Congenital, Autosomal Recessive 11 |
| UniProt Code: | Q9Y5Y6 |
| NCBI GenInfo Identifier: | 11415040 |
| NCBI Gene ID: | 6768 |
| NCBI Accession: | NP_068813 |
| UniProt Secondary Accession: | Q9Y5Y6,Q9BS01, Q9H3S0, Q9HB36, Q9HCA3, |
| UniProt Related Accession: | Q9Y5Y6 |
| Molecular Weight: | 94,770 Da |
| NCBI Full Name: | suppressor of tumorigenicity 14 protein |
| NCBI Synonym Full Names: | suppression of tumorigenicity 14 (colon carcinoma) |
| NCBI Official Symbol: | ST14Â Â |
| NCBI Official Synonym Symbols: | HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14Â Â |
| NCBI Protein Information: | suppressor of tumorigenicity 14 protein; membrane-type serine protease 1; prostamin; serine protease 14; serine protease TADG-15; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); tumor associated differentially expressed gene 15 pr |
| UniProt Protein Name: | Suppressor of tumorigenicity 14 protein |
| UniProt Synonym Protein Names: | Matriptase; Membrane-type serine protease 1; MT-SP1; Prostamin; Serine protease 14; Serine protease TADG-15; Tumor-associated differentially-expressed gene 15 protein |
| Protein Family: | Suppressor of tumorigenicity 14 protein |
| UniProt Gene Name: | ST14Â Â |
| UniProt Entry Name: | ST14_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ST14 antibody (CAB13549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse liver using ST14 antibody (CAB13549) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ST14 antibody (CAB13549). Blue: DAPI for nuclear staining. |
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