The ST6GAL1 Antibody (CAB5754) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications. By targeting the ST6GAL1 protein, this antibody enables detection and analysis in various cell types, making it suitable for investigations in glycobiology and cancer research.ST6GAL1, also known as ST6 beta-galactosamide alpha-2,6-sialyltransferase 1, is essential for the biosynthesis of sialylated glycoconjugates, which play critical roles in cell adhesion, signaling, and immune response modulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ST6GAL1 Antibody
SKU:
CAB5754
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ST6N, CDw75, SIAT1, ST6GalI, ST6GAL1
Positive Sample:
Daudi
Cellular Localization:
Golgi Apparatus, Golgi Stack Membrane, Secreted, Single-Pass Type Ii Membrane Protein.
Calculated MW:
47kDa
Observed MW:
50-70kDa
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
6480
RRID
AB_2766508
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Daudi cells, using ST6GAL1 Rabbit pAb (CAB5754) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using ST6GAL1 Rabbit pAb (CAB5754) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
