Description
Anti-STAG2 Antibody (RACO0394)
The STAG2 Recombinant Antibody (RACO0394) is a valuable tool for researchers studying the STAG2 protein and its role in cellular processes such as chromosome segregation and DNA repair. This antibody, produced using recombinant technology, exhibits high specificity and sensitivity for STAG2 and has been validated for use in various applications, including immunofluorescence and flow cytometry.STAG2, a subunit of the cohesin complex, is essential for the proper segregation of chromosomes during cell division and plays a key role in maintaining genomic integrity.
Mutations in the STAG2 gene have been linked to various cancers, making it an attractive target for therapeutic intervention and biomarker development. The STAG2 Recombinant Antibody enables researchers to accurately detect and analyze STAG2 expression in different cell types and tissues, providing valuable insights into its function and potential implications in cancer biology.
| Product Name: | STAG2 Antibody |
| Product SKU: | RACO0394 |
| Size: | 50ul |
| Host Species: | Homo sapiens (Human) |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | WB:1:500-1:5000, IF:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | A synthesized peptide derived from human SA2 |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Affinity-chromatography |
| Clonality: | Monoclonal |
| Isotype: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| Background: | Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. |
| Synonyms: | Cohesin subunit SA-2 (SCC3 homolog 2) (Stromal antigen 2), STAG2, SA2 |
 | Immunofluorescence staining of MCF7 Cells with RACO0394 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate Jurkat whole cell lysate K562 whole cell lysate All lanes SA2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 142, 146 kDa. Observed band size: 142 kDa. |
| UniProt Protein Function: | STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: Xq25 Cellular Component: actin cytoskeleton; chromatin; chromosome; chromosome, pericentric region; cytosol; intermediate filament cytoskeleton; membrane; nucleolus; nucleoplasm; nucleus; plasma membrane Molecular Function:protein binding Biological Process: negative regulation of DNA endoreduplication; protein sumoylation; sister chromatid cohesion |
| NCBI Summary: | The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Code: | Q8N3U4 |
| NCBI GenInfo Identifier: | 73621291 |
| NCBI Gene ID: | 10735 |
| NCBI Accession: | Q8N3U4.3 |
| UniProt Secondary Accession: | Q8N3U4,O00540, Q5JTI6, Q68DE9, Q9H1N8, B1AMT5, D3DTF5 |
| UniProt Related Accession: | Q8N3U4 |
| Molecular Weight: | 145,751 Da |
| NCBI Full Name: | Cohesin subunit SA-2 |
| NCBI Synonym Full Names: | stromal antigen 2 |
| NCBI Official Symbol: | STAG2Â Â |
| NCBI Official Synonym Symbols: | SA2; SA-2; SCC3B; bA517O1.1Â Â |
| NCBI Protein Information: | cohesin subunit SA-2 |
| UniProt Protein Name: | Cohesin subunit SA-2 |
| UniProt Synonym Protein Names: | SCC3 homolog 2; Stromal antigen 2 |
| Protein Family: | Cofilin |
| UniProt Gene Name: | STAG2Â Â |
| UniProt Entry Name: | STAG2_HUMAN |
