The STAR Antibody (CAB1035) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot applications. By binding to the STAR protein, researchers can effectively detect and analyze this important molecule in various cell types.STAR, also known as steroidogenic acute regulatory protein, plays a crucial role in controlling the synthesis of cholesterol and steroid hormones in the adrenal glands and gonads. Dysregulation of STAR has been linked to various metabolic disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
STAR Antibody
SKU:
CAB1035
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STARD1, StAR
Positive Sample:
HEL, K-562
Cellular Localization:
Mitochondrion.
Calculated MW:
32kDa
Observed MW:
28kDa
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
Purification Method
Affinity purification
Gene ID
6770
RRID
AB_2757895
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using StAR Rabbit pAb (CAB1035) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg StAR antibody (CAB1035). Western blot was performed from the immunoprecipitate using StAR antibody (CAB1035) at a dilution of 1:1000.
