The STX1A Antibody (CAB5570) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it an essential tool for Western blot experiments. By binding specifically to STX1A, this antibody enables precise detection and analysis in a variety of cell types, making it particularly valuable for investigations in neuroscience and neurology research.STX1A is a critical component of the synaptic machinery that regulates neurotransmitter release, making it essential for proper brain function and communication between neurons.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
STX1A Antibody
SKU:
CAB5570
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STX1, HPC-1, P35-1, SYN1A, STX1A
Positive Sample:
U-251MG, HT-1080, BT-474
Cellular Localization:
Cell Junction, Cell Membrane, Cytoplasmic Vesicle, Secreted, Single-Pass Type Iv Membrane Protein, Secretory Vesicle, Synapse, Synaptic Vesicle Membrane, Synaptosome.
Calculated MW:
33kDa
Observed MW:
33kDa
This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6804
RRID
AB_2766348
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STX1A Rabbit pAb (CAB5570) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of C6 cells using STX1A Rabbit pAb (CAB5570) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
