The SUPT5H/SPT5 Antibody (CAB9193) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the SUPT5H protein, researchers can detect and analyze its expression in a variety of cell types, making it ideal for studies in molecular biology and gene expression.SUPT5H is a key factor in the regulation of RNA polymerase II activity, playing a crucial role in the transcriptional elongation process.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SUPT5H/SPT5 Antibody
SKU:
CAB9193
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SPT5, SPT5H, Tat-CT1, SUPT5H/SPT5
Positive Sample:
U-87MG, HeLa, A-549, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
121kDa
Observed MW:
160kDa
Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of RNA metabolic process; and transcription elongation from RNA polymerase II promoter. Located in nucleoplasm. Part of DSIF complex.
Purification Method
Affinity purification
Gene ID
6829
RRID
AB_2772472
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SUPT5H/SPT5 Rabbit pAb (CAB9193) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg SUPT5H/SPT5 Rabbit pAb (CAB9193). Western blot was performed from the immunoprecipitate using SUPT5H/SPT5 Rabbit pAb (CAB9193) at a dilition of 1:500.
