The T4 (Thyroxine) ELISA Kit is a highly specialized assay designed for the precise quantitative detection of Thyroxine (T4) levels in various biological samples. Thyroxine, an important thyroid hormone produced by the thyroid gland, plays a pivotal role in regulating metabolism, growth, and development in the body. It is a crucial component of the endocrine system and influences numerous physiological processes, including energy production, protein synthesis, and temperature regulation. By measuring T4 levels with this ELISA kit, researchers can gain valuable insights into thyroid function, metabolic activity, and hormonal balance within biological samples.
Thyroxine serves as a key marker for thyroid health and can provide essential information on thyroid disorders, metabolic conditions, and other health-related concerns. The T4 ELISA Kit offers exceptional sensitivity and specificity, enabling accurate and reproducible results for researchers studying thyroid function and related physiological processes. Manufactured under strict quality control standards, this kit ensures robust performance and reliable data, making it an ideal choice for research studies focused on thyroid hormones, metabolism, and endocrine health.
Product Name:
T4 (Thyroxine) ELISA Kit
SKU:
AEES00694
Target:
T4 (Thyroxine)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with T4. During the reaction, T4 in the sample or standard competes with a fixed amount of T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to T4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
92-105
97-113
Average (%)
94
98
103
1:4
Range (%)
86-97
93-109
97-111
Average (%)
92
100
104
1:8
Range (%)
88-103
89-103
95-107
Average (%)
94
94
101
1:16
Range (%)
90-104
94-107
93-110
Average (%)
97
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.52
19.65
87.25
9.82
19.15
88.57
Standard deviation
0.59
1.13
2.62
0.68
0.83
3.36
C V (%)
6.2
5.75
3.0
6.92
4.33
3.79
Sample volume:
50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of T4 concentrations in .
Specificity:
This kit recognizes T4 in samples. No significant cross-reactivity or interference between T4 and analogues was observed.
